Doaa A. Zaky
Cairo University, Egypt
Title: Novel gut barrier modulatory effect of Vildagliptin in amelioration of bacterial translocation in cholestatic rats.
Biography
Biography: Doaa A. Zaky
Abstract
Intestinal barrier dysfunction is frequently implicated in the etiology of septic complications following obstructive jaundice. Th e present study was conducted to investigate the potential gut barrier modulating effect of Vildagliptin (VLD), a dipeptidyl peptidase-IV (DPP-IV) inhibitor, on common bile duct ligation (CBDL) in rats. VLD (10 mg/ kg/d; p.o) was administered for 10 successive days aft er CBDL in male Sprague-Dawley rats. VLD reduced portal endotoxemia induced by CBDL and suppressed rectal temperature elevation, body weight loss and colon and spleen indices to diff erent extents. Moreover, it reversed villous blunting, low villous density, mucosal thinning and infl ammatory cell infi ltration noticed microscopically in the ileal segments of the CBDL rats. Th ese events were accompanied by the reduction of intestinal myeloperoxidase activity and lipid peroxidation, the elevation of the major anti-oxidant molecule, glutathione, as well as the normalization of ileal nitric oxide level. Furthermore, ileal DPP-IV was inhibited in association with the rise in ileal glucagon-like peptide-1 (GLP-1) and insulin-like growth factor-1 (IGF-1) levels in VLD treated animals. Th e current fi ndings suggest that VLD anti-oxidant, antiinfl ammatory, as well as enhancements of incretin and IGF-1 account for the hardwearing of the intestinal barrier that functions competently in the absence of the supporting bile elements.